pollen culture

In anther and pollen culture, using the low temperature treating anthotaxy ( 4, 1~ 2day) before being inoculated and high temperature culturing ( 33 1~ 2day) after being inoculated can promote the embryogenesis and increase embryoid rate.

对于花药培养和花粉培养，采用低温预处理花序（4℃下1～2d）和高温预培养（33℃下1～2d），均能促进胚状体的形成，提高产胚率。

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With the method of anther and pollen culture, it can be got double haploid plant. Therefore, it may greatly shorten the time of purification in the breeding.

若象大白菜、甘兰型油菜那样通过游离小孢子培养的方法获得双单倍体（Double Haploid；DH）植株，可大大缩短纯化菜心品种所需的时间并减少工作量。

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This article summarized the application of biotechnology breeding in fruit trees from embryo culture, anther and pollen culture, protoplast culture and cell fusion, gene engineering breeding. The direction which needs further investigation was also discussed.

本文从胚培育种、花药和花粉培养育种、原生质体培养与细胞融合、基因工程育种四个方面综述了生物技术在果树育种上的运用，并对其未来的发展作了展望。

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